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1 5th Department of Internal Medicine - Oncology/Hematology, Hospital Hietzing, Vienna, Austria.
2 Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.
3 Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria.
4 5th Department of Internal Medicine - Oncology/Hematology, Hospital Hietzing, Vienna, Austria; Ludwig Boltzmann Institute for Clinical Oncology, Vienna, Austria. Electronic address: klaus.geissler@wienkav.at.
1 5th Department of Internal Medicine - Oncology/Hematology, Hospital Hietzing, Vienna, Austria.
2 Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.
3 Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria.
4 5th Department of Internal Medicine - Oncology/Hematology, Hospital Hietzing, Vienna, Austria; Ludwig Boltzmann Institute for Clinical Oncology, Vienna, Austria. Electronic address: klaus.geissler@wienkav.at.
Unstimulated methylcellulose cultures in 25 myelofibrosis (MF) patients were performed to better understand the role of cytokines in the proliferation of MF cells. Compared to controls MF patients show a variable but highly increased spontaneous CFU-GM formation (66 vs 4.8/10(5) PBMNC). There was a marked reduction of autonomous CFU-GM growth by the cytokine-synthesis-inhibiting molecule IL-10 as well as by antibodies against GM-CSF whereas antibodies against IL-3, G-CSF, M-CSF and IL-1β showed heterogeneous effects. Spontaneous CFU-GM growth >100/10(5) PBMNC predicted shorter survival. Constitutive release of GM-CSF seems to contribute to proliferation of MF cells in vitro and possibly in vivo.
