Photosynthetic adaptation of pea plants grown at different light intensities: State 1 - State 2 transitions and associated chlorophyll fluorescence changes

Abstract

A study of pea plants grown at different light intensities has been made. Using a leaf oxygen electrode, it was shown that plants grown under low light intensities had lower saturated rates of photosynthesis than high-light-grown plants however, at low light intensities the photosynthetic rates were similar for both types of plants. State 1- State 2 transitions have been monitored with attached leaves using a modulated fluorescence technique. It is shown that peas grown under low light intensities (20 W m(-2)) had a faster State 1 to State 2 transition when compared with medium-(50 W m(-2)) and high-(70 W m(-2)) light-grown plants. Measurement of fast-fluorescence-induction curves in the absence of 3-(3',4'-dichlorophenyl)-1,1-dimethylurea (DCMU) have shown that low-light plants are, when in State 1, more effective at using Photosystem-two (PSII) light to reduce their plastoquinone pool than high-light plants. Transition from State 1 to State 2 for all plants led to a decrease in the reduction level of the plastoquinone pool inidcating that the transition had increased electron flow through Photosystem one (PSI) relative to PSII. Analyses of fast fluorescence induction in the presence of DCMU indicate that low-light-grown plants have a higher PSII-α/PSII-β ratio than high-light-grown plants. Such a difference is in line with the increase in the PSII/PSI ratio of low-light plants and is reflected in their high chlorophyll b/chlorophyll a ratio and their larger appressed to non-appressed thylakoid-membrane areas. It is suggested that these two latter factors give rise to the faster State 1 - State 2 transitions in low-light plants.