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. 1983 Jan;64(2):117-8.
doi: 10.1007/BF00272717.

Detection of two different nuclear genomes in parasexual hybrids by ribosomal RNA gene analysis

H Uchimiya  1 , T Ohgawara, H Kato, T Akiyama, H Harada, M Sugiura
Affiliations

Detection of two different nuclear genomes in parasexual hybrids by ribosomal RNA gene analysis

H Uchimiya et al. Theor Appl Genet. 1983 Jan.

Abstract

Restriction endonucleases discriminated between the rDNAs contained in callus tissues derived from Nicotiana glauca, N. langsdorffii, and their somatic hybrids produced by protoplast fusion. With XbaI, a single repeat fragment of 7.5 × 10(6) daltons was produced from N. glauca rDNA compared to a single repeat fragment of 4.2 × 10(6) daltons produced from N. langsdorffii rDNA. Both kinds of XbaI fragments were found in somatic hybrids.

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