Two dimensional analysis of chloroplast proteins from normal and cytoplasmic male sterile Brassica napus

Abstract

Stromal and thylakoid proteins isolated from normal (N) and cytoplasmic male sterile (cms) lines of Brassica napus have been compared using a two dimensional gel separation. It has been shown that: 1) stromal compartments of the two lines were very similar; 2) although there was extensive homology between protein maps of thylakoids isolated from the two lines, these could be distinguished by the spots corresponding to the β subunits of the coupling factor CF1 from the ATPase complex.