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. 2014 Mar;8(2):131-4.
doi: 10.1111/irv.12210. Epub 2013 Nov 24.

Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon

Sebastien Kenmoe  1 , Patrice Tchendjou, Suzie Moyo Tetang, Tatiana Mossus, Mohammed Njankouo Ripa, Marlène Guillet, Anfumbom Kfutwah, Richard Njouom
Affiliations

Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon

Sebastien Kenmoe et al. Influenza Other Respir Viruses. 2014 Mar.

Abstract

The performance of SD Bioline rapid antigen test (RAT) was evaluated using real-time reverse transcription polymerase chain reaction (rRT-PCR) as gold standard. A total of 718 nasal swabs, including 102 rRT-PCR positive and 616 rRT-PCR negative swabs, were tested. RAT demonstrates a sensitivity of 29·4% with a specificity of 100%. The positivity rate of RAT was highly associated with lower cycle threshold (Ct) values (P < 0·0001). The excellent specificity of the RAT allowed for the rapid identification of influenza cases. However, negative results should be verified by rRT-PCR test because of limitations observed in sensitivity.

Keywords: Cameroon; SD Bioline; influenza; sensitivity; specificity.

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Figures

Figure 1
Figure 1
Comparison of cycle threshold (Ct) values for influenza real-time reverse transcription polymerase chain reaction positive specimens (n = 102) with negative and positive rapid antigen test (RAT) result. Within each box plot, the centre line represents the median, while the top and bottom borders mark the 75th and 25th percentiles, respectively. Whiskers represent the minimum and maximum values, except for an extreme outlier, which is indicated by an open circle. Boxplots were produced using the R program version 2.15.1.
Figure 2
Figure 2
Number of influenza-like illness specimens (bars) and the rate of influenza positive specimens (line) from October 2011 to November 2012 in Yaounde, Cameroon.
See this image and copyright information in PMC

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References

    1. Simmerman JM, Chittaganpitch M, Erdman D, Sawatwong P, Uyeki TM, Dowell SF. Field performance and new uses of rapid influenza testing in Thailand. Int J Infect Dis. 2007;11:166–171. - PubMed
    1. Yoo Y, Sohn JW, Park DW, et al. Clinical evaluation of the SD Bioline influenza virus antigen test for rapid detection of influenza viruses A and B in children and adults during the influenza season. Clin Vaccine Immunol. 2007;14:1050–1052. - PMC - PubMed
    1. Agoritsas K, Mack K, Bonsu BK, Goodman D, Salamon D, Marcon MJ. Evaluation of the Quidel QuickVue test for detection of influenza A and B viruses in the pediatric emergency medicine setting by use of three specimen collection methods. J Clin Microbiol. 2006;44:2638–2641. - PMC - PubMed
    1. Rodriguez WJ, Schwartz RH, Thorne MM. Evaluation of diagnostic tests for influenza in a pediatric practice. Pediatr Infect Dis J. 2002;21:193–196. - PubMed
    1. Druce J, Tran T, Kelly H, et al. Laboratory diagnosis and surveillance of human respiratory viruses by PCR in Victoria, Australia, 2002–2003. J Med Virol. 2005;75:122–129. - PMC - PubMed

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