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. 2014 Jan;11(1):47-50.
doi: 10.1038/nmeth.2734. Epub 2013 Nov 24.

A quantitative liposome microarray to systematically characterize protein-lipid interactions

Antoine-Emmanuel Saliba  1 , Ivana Vonkova  1 , Stefano Ceschia  2 , Greg M Findlay  3 , Kenji Maeda  2 , Christian Tischer  4 , Samy Deghou  2 , Vera van Noort  2 , Peer Bork  2 , Tony Pawson  5 , Jan Ellenberg  6 , Anne-Claude Gavin  2
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A quantitative liposome microarray to systematically characterize protein-lipid interactions

Antoine-Emmanuel Saliba et al. Nat Methods. 2014 Jan.

Abstract

Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.

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References

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