Mouse Single Oral Dose Toxicity Study of DHU001, a Polyherbal Formula

Abstract

This study was conducted to obtain acute information of the oral dose toxicity of DHU001, a polyherbal formula in male and female mice. In order to calculated 50% lethal dose (LD50) and approximate lethal dose (LD) , test material was once orally administered to male and female ICR mice at dose levels of 2000, 1000, 500, 250 and 0 (vehicle control) ml/kg (body weight) . The mortality and changes on body weight, clinical signs, gross observation, organ weight and histopathology of principle organs were monitored 14 days after treatment with DHU001. We could not find any mortalities, DHU001 treatment-related clinical signs, changes on the body and organ weights, gross and histopathological findings. The results obtained in this study suggest that LD50 and approximate LD in mice after single oral dose of DHU001 were considered over 2000 mg/kg in both female and male mice.

Keywords: DHU001; Histopathology; Mice; Polyherbal formula; Single oral dose toxicity.

Fig. 1.. Body weight changes in male mice after once orally dose of DHU001. No significant changes were detected in all DHU001 treated groups as compared with vehicle control. Before means 1 day before administration; Day 0 means at administration; All animals at sacrifice and Day 0 overnight fasted; Values are expressed as mean ± S.D. of five mice.

Fig. 2.. Body weight changes in female mice after once orally dose of DHU001. No significant changes were detected in all DHU001 treated groups as compared with vehicle control. Before means 1 day before administration; Day 0 means at administration; All animals at sacrifice and Day 0 overnight fasted; Values are expressed as mean ± S.D. of five mice.