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. 1986 Sep;22(9):557-60.
doi: 10.1007/BF02621143.

Establishment and characterization of a thymic medullary epithelial cell clone

E F Potworowski, F Turcotte, C Beauchemin, P Hugo, M G Zelechowska

Establishment and characterization of a thymic medullary epithelial cell clone

E F Potworowski et al. In Vitro Cell Dev Biol. 1986 Sep.

Abstract

Thymic stromal cells were cultured in conditions which select for epithelial cells. These were then transformed in vitro by contact with N-methyl-N'-nitro-N-nitrosoguanidine and cloned at limit dilution. One of the clones was characterized as being of medullary origin on the basis of its reactivity with a battery of antibodies previously shown to distinguish cortical from medullary thymic epithelial cells. The importance of this clone lies in the potential it offers to delineate how various T cell subpopulations acquire their distinct markers and function within the thymus.

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