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. 2014 Jan:51:145-9.
doi: 10.1016/j.peptides.2013.11.016. Epub 2013 Nov 27.

Intravenous infusion of gastrin-releasing peptide-27 and bombesin in rats reveals differential effects on meal size and intermeal interval length

Martha C Washington  1 , Sarah Salyer  1 , Amnah H Aglan  2 , Ayman I Sayegh  3
Affiliations

Intravenous infusion of gastrin-releasing peptide-27 and bombesin in rats reveals differential effects on meal size and intermeal interval length

Martha C Washington et al. Peptides. 2014 Jan.

Abstract

We have previously shown that the intraperitoneal (i.p.) administration of gastrin-releasing peptide-27 (GRP-27) or bombesin (BN) (at 0.21, 0.41 and 1.03nmol/kg) reduces meal size (MS) and prolongs the intermeal interval (IMI). Here, we hypothesized that the intravenous (i.v.) administration of the same doses of GRP-27 and BN will be as effective as the i.p. administration in evoking these feeding responses. To test this hypothesis, we administered GRP-27 and BN i.v. and measured first MS (10% sucrose), IMI, satiety ratio (SR, IMI/MS) and second MS in overnight food-deprived but not water-deprived male Sprague Dawley rats. We found that (1) only GRP-27 reduced the first MS, (2) BN prolonged the IMI, (3) GRP-27 and BN increased the SR and (4) only BN reduced the size of the second meal. Contrary to our hypothesis, the i.v. administration of GRP-27 and BN affected the MS and IMI differently than did the i.p. administration. In conclusion, this pharmacological study suggests that the MS and IMI are regulated at different sites.

Keywords: Bombesin; GRP; Gastrin-releasing peptide; Gastrointestinal tract; Intermeal interval; Meal size.

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Figures

Figure 1
Figure 1. Effect of the intravenous infusion of gastrin-releasing peptide-27 and bombesin on the first meal size
Gastrin-releasing peptide-27 (GRP-27) and bombesin (BN) (0, 0.21, 0.41 and 1.03 nmol/kg) were infused in the femoral vein of overnight food-deprived Sprague Dawley rats (n=8), and the first meal size (MS, 10% sucrose) was determined. Only GRP-27 (all doses) reduced the MS relative to the effect of the saline vehicle (* denotes the significance, p<0.05). Black bar: saline, empty bars: GRP-27, gray bars: BN
Figure 2
Figure 2. Effect of the intravenous infusion of gastrin-releasing peptide-27 and bombesin on the length of the intermeal interval
Gastrin-releasing peptide-27 (GRP-27) and bombesin (BN) (0, 0.21, 0.41 and 1.03 nmol/kg) were infused in the femoral vein of overnight food-deprived Sprague Dawley rats (n=8), and the time between the first and the second meals (intermeal interval, IMI) was determined. GRP-27 (1.03 nmol/kg) and BN (all doses) prolonged the IMI relative to the effect of the saline vehicle (* denotes the significance, p<0.05). Black bar: saline, empty bars: GRP-27, gray bars: BN
Figure 3
Figure 3. Effect of the intravenous infusion of gastrin-releasing peptide-27 and bombesin on the satiety ratio
Gastrin-releasing peptide-27 (GRP-27) and bombesin (BN) (0, 0.21, 0.41 and 1.03 nmol/kg) were infused in the femoral vein of overnight food-deprived Sprague Dawley rats (n=8), and the satiety ratio (SR; the time between two meals (the intermeal interval in minutes) divided by the first meal size in ml was determined. GRP-27 (1.03 nmol/kg) and BN (0.21 and 1.03 nmol/kg) increased the SR relative to the effect of the saline vehicle (* denotes the significance, p<0.05), and BN at 1.03 nmol/kg increased this ratio more than the same dose of GRP-27 († denotes the significance, p<0.05). Black bar: saline, empty bars: GRP-27, gray bars: BN
Figure 4
Figure 4. Effect of the intravenous infusion of gastrin-releasing peptide-27 and bombesin on the size of the second meal
Gastrin-releasing peptide-27 (GRP-27) and bombesin (BN) (0, 0.21, 0.41 and 1.03 nmol/kg) were infused in the femoral vein of overnight food-deprived Sprague Dawley rats (n=8), and the second meal size (MS, 10% sucrose) was determined. Only BN (0.21 nmol/kg) reduced the second MS relative to the effect of the saline vehicle (* denotes the significance, p<0.05). Black bar: saline, empty bars: GRP-27, gray bars: BN
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