Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Jan:51:145-9.
doi: 10.1016/j.peptides.2013.11.016. Epub 2013 Nov 27.

Intravenous infusion of gastrin-releasing peptide-27 and bombesin in rats reveals differential effects on meal size and intermeal interval length

Martha C Washington  1 , Sarah Salyer  1 , Amnah H Aglan  2 , Ayman I Sayegh  3
Affiliations

Intravenous infusion of gastrin-releasing peptide-27 and bombesin in rats reveals differential effects on meal size and intermeal interval length

Martha C Washington et al. Peptides. 2014 Jan.

Abstract

We have previously shown that the intraperitoneal (i.p.) administration of gastrin-releasing peptide-27 (GRP-27) or bombesin (BN) (at 0.21, 0.41 and 1.03nmol/kg) reduces meal size (MS) and prolongs the intermeal interval (IMI). Here, we hypothesized that the intravenous (i.v.) administration of the same doses of GRP-27 and BN will be as effective as the i.p. administration in evoking these feeding responses. To test this hypothesis, we administered GRP-27 and BN i.v. and measured first MS (10% sucrose), IMI, satiety ratio (SR, IMI/MS) and second MS in overnight food-deprived but not water-deprived male Sprague Dawley rats. We found that (1) only GRP-27 reduced the first MS, (2) BN prolonged the IMI, (3) GRP-27 and BN increased the SR and (4) only BN reduced the size of the second meal. Contrary to our hypothesis, the i.v. administration of GRP-27 and BN affected the MS and IMI differently than did the i.p. administration. In conclusion, this pharmacological study suggests that the MS and IMI are regulated at different sites.

Keywords: Bombesin; GRP; Gastrin-releasing peptide; Gastrointestinal tract; Intermeal interval; Meal size.

PubMed Disclaimer

Figures

Figure 1
Figure 1. Effect of the intravenous infusion of gastrin-releasing peptide-27 and bombesin on the first meal size
Gastrin-releasing peptide-27 (GRP-27) and bombesin (BN) (0, 0.21, 0.41 and 1.03 nmol/kg) were infused in the femoral vein of overnight food-deprived Sprague Dawley rats (n=8), and the first meal size (MS, 10% sucrose) was determined. Only GRP-27 (all doses) reduced the MS relative to the effect of the saline vehicle (* denotes the significance, p<0.05). Black bar: saline, empty bars: GRP-27, gray bars: BN
Figure 2
Figure 2. Effect of the intravenous infusion of gastrin-releasing peptide-27 and bombesin on the length of the intermeal interval
Gastrin-releasing peptide-27 (GRP-27) and bombesin (BN) (0, 0.21, 0.41 and 1.03 nmol/kg) were infused in the femoral vein of overnight food-deprived Sprague Dawley rats (n=8), and the time between the first and the second meals (intermeal interval, IMI) was determined. GRP-27 (1.03 nmol/kg) and BN (all doses) prolonged the IMI relative to the effect of the saline vehicle (* denotes the significance, p<0.05). Black bar: saline, empty bars: GRP-27, gray bars: BN
Figure 3
Figure 3. Effect of the intravenous infusion of gastrin-releasing peptide-27 and bombesin on the satiety ratio
Gastrin-releasing peptide-27 (GRP-27) and bombesin (BN) (0, 0.21, 0.41 and 1.03 nmol/kg) were infused in the femoral vein of overnight food-deprived Sprague Dawley rats (n=8), and the satiety ratio (SR; the time between two meals (the intermeal interval in minutes) divided by the first meal size in ml was determined. GRP-27 (1.03 nmol/kg) and BN (0.21 and 1.03 nmol/kg) increased the SR relative to the effect of the saline vehicle (* denotes the significance, p<0.05), and BN at 1.03 nmol/kg increased this ratio more than the same dose of GRP-27 († denotes the significance, p<0.05). Black bar: saline, empty bars: GRP-27, gray bars: BN
Figure 4
Figure 4. Effect of the intravenous infusion of gastrin-releasing peptide-27 and bombesin on the size of the second meal
Gastrin-releasing peptide-27 (GRP-27) and bombesin (BN) (0, 0.21, 0.41 and 1.03 nmol/kg) were infused in the femoral vein of overnight food-deprived Sprague Dawley rats (n=8), and the second meal size (MS, 10% sucrose) was determined. Only BN (0.21 nmol/kg) reduced the second MS relative to the effect of the saline vehicle (* denotes the significance, p<0.05). Black bar: saline, empty bars: GRP-27, gray bars: BN
See this image and copyright information in PMC

References

    1. Corp ES, Melville LD, Greenberg D, Gibbs J, Smith GP. Effect of fourth ventricular neuropeptide Y and peptide YY on ingestive and other behaviors. Am J Physiol. 1990;259:R317–23. - PubMed
    1. Ekblad E, Ekman R, Hakanson R, Sundler F. Return of nerve fibers containing gastrin-releasing peptide in rat small intestine after local removal of myenteric ganglia. Neuroscience. 1988;24:309–19. - PubMed
    1. Furness JB, Miller AS, Costa M. The presence and possible roles of bombesin-like peptides in enteric neurons. Ann N Y Acad Sci. 1988;547:76–82. - PubMed
    1. Gibbs J. Effect of bombesin on feeding behavior. Life Sci. 1985;37:147–53. - PubMed
    1. Gibbs J, Fauser DJ, Rowe EA, Rolls BJ, Rolls ET, Maddison SP. Bombesin suppresses feeding in rats. Nature. 1979;282:208–10. - PubMed

Publication types