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. 2013 Oct 30;44(2):595-605.
doi: 10.1590/S1517-83822013000200040. eCollection 2013.

In situ biosurfactant production and hydrocarbon removal by Pseudomonas putida CB-100 in bioaugmented and biostimulated oil-contaminated soil

Martínez-Toledo Ángeles  1 , Rodríguez-Vázquez Refugio
Affiliations

In situ biosurfactant production and hydrocarbon removal by Pseudomonas putida CB-100 in bioaugmented and biostimulated oil-contaminated soil

Martínez-Toledo Ángeles et al. Braz J Microbiol. .

Abstract

In situ biosurfactant (rhamnolipid) production by Pseudomonas putida CB-100 was achieved during a bioaugmented and biostimulated treatment to remove hydrocarbons from aged contaminated soil from oil well drilling operations. Rhamnolipid production and contaminant removal were determined for several treatments of irradiated and non-irradiated soils: nutrient addition (nitrogen and phosphorus), P. putida addition, and addition of both (P. putida and nutrients). The results were compared against a control treatment that consisted of adding only sterilized water to the soils. In treatment with native microorganisms (non-irradiated soils) supplemented with P. putida, the removal of total petroleum hydrocarbons (TPH) was 40.6%, the rhamnolipid production was 1.54 mg/kg, and a surface tension of 64 mN/m was observed as well as a negative correlation (R = -0.54; p < 0.019) between TPH concentration (mg/kg) and surface tension (mN/m), When both bacteria and nutrients were involved, TPH levels were lowered to 33.7%, and biosurfactant production and surface tension were 2.03 mg/kg and 67.3 mN/m, respectively. In irradiated soil treated with P. putida, TPH removal was 24.5% with rhamnolipid generation of 1.79 mg/kg and 65.6 mN/m of surface tension, and a correlation between bacterial growth and biosurfactant production (R = -0.64; p < 0.009) was observed. When the nutrients and P. putida were added, TPH removal was 61.1%, 1.85 mg/kg of biosurfactants were produced, and the surface tension was 55.6 mN/m. In summary, in irradiated and non-irradiated soils, in situ rhamnolipid production by P. putida enhanced TPH decontamination of the soil.

Keywords: P. putida; bioremediation; irradiated soil; rhamnolipids; total petroleum hydrocarbons.

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Figures

Figure 1
Figure 1
Soil sampling map.
Figure 2
Figure 2
Instantaneous CO2 production in A) irradiated soil and B) non-irradiated soil.
Figure 3
Figure 3
Bacterial growth in A) irradiated soil and B) non-irradiated soil.
Figure 4
Figure 4
Fungal growth in A) irradiated soil and B) non-irradiated soil.
Figure 5
Figure 5
Rhamnolipid concentration vs. TPH removal from BioauT-BiostT in A) irradiated soil and B) non-irradiated soil.
See this image and copyright information in PMC

References

    1. Aitken M, Stringfellow WT, Nagel R, Kazunga CH, Chen S. Characteristic of phenanthrene-degrading bacteria isolated from soils contaminated with polycyclic aromatic hydrocarbons. Can J Microbiol. 1998;44:743–752. - PubMed
    1. Amézcua-Allieri MA, Lead JR, Meléndez-Estrada J, Rodríguez-Vázquez R. Phenanthrene removal in selected mexican soils by the fungus Penicillium frequentans: Role of C:N ratio and water content. Soil Sediment Contam. 2003;12:387–399.
    1. Amézcua-Vega C, Ferrera-Cerrato R, Esparza García F, Ríos-Leal E, Rodríguez-Vázquez R. Effect of combined nutrients on biosurfactants produced by Pseudomonas putida. J Environ Sci Heal A. 2004;39:2983–2991. - PubMed
    1. Arce-Ortega JM, Rodríguez-Vázquez R, Rojas-Avelizapa NG. Identification of recalcitrant hydrocarbons present in drilling waste polluted soil. J Environ Sci Heal A. 2004;39:1535–1545. - PubMed
    1. Banks MK, Govindaraju RS, Schwab AP, Kulakow R, Finn J. Phytoremediation of Hydrocarbon-Contaminated Soil. Lewis Publishers CRC press LLC; USA: 2000.

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