Electrophoretic identification of fusion proteins expressed in single recombinant lambda-bacteriophage plaques

Abstract

Sufficient fusion protein is present in single plaques produced by lytic, recombinant lambda bacteriophage to be detected by Coomassie blue staining following electrophoresis on sodium dodecyl sulfate-polyacrylamide gels. Agar plugs containing single plaques can be loaded directly onto the stacking gel thus avoiding the need for extensive sample preparation.