Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Oct 29;4(11):2673-82.
doi: 10.1364/BOE.4.002673. eCollection 2013.

A simple and rapid detection of tissue adhesive-induced biochemical changes in cells and DNA using Raman spectroscopy

Gyeong Bok Jung  1 , Young Ju Lee, Gihyun Lee, Hun-Kuk Park
Affiliations

A simple and rapid detection of tissue adhesive-induced biochemical changes in cells and DNA using Raman spectroscopy

Gyeong Bok Jung et al. Biomed Opt Express. .

Abstract

We demonstrate a cytotoxicity evaluation of tissue adhesive using Raman spectroscopy. This method allows for quantitative, label-free, non-invasive and rapid monitoring of the biochemical changes of cells following tissue adhesive treatment. Here, we show the biochemical property changes in mouse fibroblast L929 cells and cellular DNA following tissue adhesive (Dermabond) treatment using Raman spectroscopy. The Raman band intensities were significantly decreased when the cells were treated with Dermabond as compared to control cells. These results suggest denaturation and conformational changes in proteins and degradation of DNA related to cell death. To support these conclusions, conventional cytotoxicity assays such as WST, LIVE/DEAD, and TUNEL were carried out, and the results were in agreement with the Raman results. Thus, Raman spectroscopy analysis not only distinguishes between viable and damaged cells, but can also be used for identification and quantification of a cytotoxicity of tissue adhesive, which based on the cellular biochemical and structural changes at a molecular level. Therefore, we suggest that this method could be used for cytotoxic evaluation of tissue adhesives by rapid and sensitive detection of cellular changes.

Keywords: (170.0170) Medical optics and biotechnology; (170.1530) Cell analysis; (170.5660) Raman spectroscopy; (300.6450) Spectroscopy, Raman.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Averaged Raman spectra of L929 cells: (a) Control and (b) Dermabond-treated. The spectrum (c) shows the spectral differences of control cells and cells treated with Dermabond tissue adhesive.
Fig. 2
Fig. 2
Relative intensities of the Raman peaks for control cells and cells treated with Dermabond tissue adhesive.
Fig. 3
Fig. 3
Averaged Raman spectra of DNA: (a) Control and (b) Dermabond-treated. The spectrum (c) shows the spectral differences of control DNA and DNA treated with Dermabond tissue adhesive.
Fig. 4
Fig. 4
Relative intensities of the Raman peaks for control DNA and DNA treated with Dermabond tissue adhesive.
Fig. 5
Fig. 5
WST and LIVE/DEAD viability/cytotoxicity assays were used to determine cytotoxicity after Dermabond tissue adhesive treatment. Mouse L929 fibroblasts (1 × 105 cells) were seeded in 12-well plates and treated with Dermabond. After a 24 hr incubation, cytotoxicity was analyzed using the (a) WST assay and (b) LIVE/DEAD viability/cytotoxicity assay.
Fig. 6
Fig. 6
Evaluation of apoptotic cell death in Dermabond tissue adhesive-treated cells by TUNEL assay. The TUNEL assay was performed in (a) negative control, (b) Dermabond-treated L9292 cells and (c) positive control.
See this image and copyright information in PMC

References

    1. Applebaum J. S., Zalut T., Applebaum D., “The use of tissue adhesion for traumatic laceration repair in the emergency department,” Ann. Emerg. Med. 22(7), 1190–1192 (1993).10.1016/S0196-0644(05)80988-6 - DOI - PubMed
    1. Leahey A. B., Gottsch J. D., Stark W. J., “Clinical experience with N-butyl cyanoacrylate (Nexacryl) tissue adhesive,” Ophthalmology 100(2), 173–180 (1993). - PubMed
    1. Blanco L. P., “Lip suture with isobutyl cyanoacrylate,” Endod. Dent. Traumatol. 10(1), 15–18 (1994).10.1111/j.1600-9657.1994.tb00592.x - DOI - PubMed
    1. Leggat P. A., Smith D. R., Kedjarune U., “Surgical applications of cyanoacrylate adhesives: a review of toxicity,” ANZ J. Surg. 77(4), 209–213 (2007).10.1111/j.1445-2197.2007.04020.x - DOI - PubMed
    1. Vote B. J., Elder M. J., “Cyanoacrylate glue for corneal perforations: a description of a surgical technique and a review of the literature,” Clin. Experiment. Ophthalmol. 28(6), 437–442 (2000).10.1046/j.1442-9071.2000.00351.x - DOI - PubMed

LinkOut - more resources