doi: 10.1016/0378-1119(86)90056-9.
Direct identification of small sequence changes in chromosomal DNA
- PMID: 2429900
- DOI: 10.1016/0378-1119(86)90056-9
Item in Clipboard
Direct identification of small sequence changes in chromosomal DNA
Gene.
1986.
Free article
Abstract
Dideoxynucleotide chain termination sequencing has been applied directly to genomic DNA templates by annealing radiolabeled oligodeoxynucleotide primers to unique sites in total yeast DNA and extending with avian myoblastosis virus (AMV) reverse transcriptase. The technique is used here to confirm the introduction of selectively altered tRNA genes into the Saccharomyces cerevisiae genome by gene replacement.
Similar articles
-
Sequence of a yeast DNA fragment containing a chromosomal replicator and a tRNA Glu 3 gene.Nucleic Acids Res. 1981 Jun 25;9(12):2949-59. doi: 10.1093/nar/9.12.2949. Nucleic Acids Res. 1981. PMID: 6269065 Free PMC article.
-
The methionine initiator tRNA genes of yeast.Gene. 1986;41(2-3):343-8. doi: 10.1016/0378-1119(86)90118-6. Gene. 1986. PMID: 3011608
-
Characterization of the yeast tRNA Ser genomic organization and DNA sequence.Nucleic Acids Res. 1981 Feb 25;9(4):921-34. doi: 10.1093/nar/9.4.921. Nucleic Acids Res. 1981. PMID: 7015287 Free PMC article.
-
Three regions of a yeast tRNALeu3 gene promote RNA polymerase III transcription.J Biol Chem. 1984 May 10;259(9):5990-4. J Biol Chem. 1984. PMID: 6371014
-
Complementary-addressed (sequence-specific) modification of nucleic acids.Prog Nucleic Acid Res Mol Biol. 1985;32:291-320. doi: 10.1016/s0079-6603(08)60352-9. Prog Nucleic Acid Res Mol Biol. 1985. PMID: 2418466 Review. No abstract available.
Cited by
-
Comparison of tRNA gene transcription complexes formed in vitro and in nuclei.Mol Cell Biol. 1987 Sep;7(9):3212-20. doi: 10.1128/mcb.7.9.3212-3220.1987. Mol Cell Biol. 1987. PMID: 3313009 Free PMC article.
-
Effects of tRNA-intron structure on cleavage of precursor tRNAs by RNase P from Saccharomyces cerevisiae.Nucleic Acids Res. 1988 Mar 25;16(6):2537-52. doi: 10.1093/nar/16.6.2537. Nucleic Acids Res. 1988. PMID: 3283703 Free PMC article.
-
Identification of mar mutations in herpes simplex virus type 1 glycoprotein B which alter antigenic structure and function in virus penetration.J Virol. 1989 Feb;63(2):730-8. doi: 10.1128/JVI.63.2.730-738.1989. J Virol. 1989. PMID: 2463380 Free PMC article.
-
Partial characterization of an RNA component that copurifies with Saccharomyces cerevisiae RNase P.Mol Cell Biol. 1989 Jun;9(6):2536-43. doi: 10.1128/mcb.9.6.2536-2543.1989. Mol Cell Biol. 1989. PMID: 2503708 Free PMC article.
-
Genomic footprinting of a yeast tRNA gene reveals stable complexes over the 5'-flanking region.Mol Cell Biol. 1989 Aug;9(8):3244-52. doi: 10.1128/mcb.9.8.3244-3252.1989. Mol Cell Biol. 1989. PMID: 2677668 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
