Analysis of the biological functions and fine specificity of (T,G)-A--L specific T cell clones

Abstract

Two T cell lines, TPB1 and TPB2, specific for the synthetic polypeptide antigen (T,G)-A--L, were established from (T,G)-A--L primed lymph node cells of C3H.SW(H-2b) mice. Both lines proliferated in the presence of (T,G)-A--L, helped in antibody production in vitro, and secreted IL2 upon stimulation with antigen. The lines differed in the fine specificity of their responses to antigenic stimulation. The line with the broader specificity TPB2 was cloned by limiting dilution, and its derived clones were analyzed. No efficient manifestation of both proliferative activity and helper function could be detected in a single clone. Most of the clones were highly specific to (T,G)-A--L, although 2 of them cross-reacted with the closely related polypeptide (Phe,G)-A--L. Individual clones could trigger B cells for the production of antibodies of the IgM and IgG classes. All helper clones secreted (T,G)-A--L specific helper factors. No correlation was found between efficient secretion of IL2 by the clones and their other biological functions.