Copulation releaser pheromone in body scales of female whitemarked tussock moth,Orgyia leucostigma (Lepidoptera: Lymantriidae): Identification and behavioral role

Abstract

The copulatory behavior of the male whitemarked tussock moth,Orgyia leucostigma, was released by extracts of female body scales applied to rubber septum models baited with a female sex pheromone gland. The major compounds in the scale extracts were identified by GC-MS as a series ofn-alkanes from C-21 to C-29. Of these,n-tricosane,n-tetracosane,n-pentacosane, andn-heptacosane, applied at 10 ng/septum, caused significantly more males to attempt copulation than hexane-treated controls. Mixtures of then-alkanes, resembling the composition in the scale extracts, were no better than the two most active alkanes,n-tetracosane andn-pentacosane, alone. The releaser effect of then-alkanes was dose dependent. EAG responses to the identifiedn-alkanes were small suggesting, along with the behavioral observations, that their perception occurred at very close range. Other factors releasing male copulatory behavior are discussed.