doi: 10.1007/BF00021328.
Bleomycin resistance: a new dominant selectable marker for plant cell transformation
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- PMID: 24302302
- DOI: 10.1007/BF00021328
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Bleomycin resistance: a new dominant selectable marker for plant cell transformation
Plant Mol Biol.
1986 May.
Abstract
Plant cells are sensitive to the antibiotic bleomycin, a DNA damaging glycopeptide. A bleomycin resistance determinant, located on transposon Tn5 and functional in bacteria, has been cloned in a plant expression vector and introduced into Nicotiana plumbaginifolia using Agrobacterium tumefaciens. The expression of this determinant in plant cells confers resistance to bleomycin and allows selection of transformed plant cells.
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