Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Jul;75(4):493-5.
doi: 10.4103/0250-474X.119826.

A Validated High-performance Liquid Chromatograhy Method for Estimation of Ferulic Acid in Asafoetida and Polyherbal Preparation

J A Kareparamban  1 , P H Nikam, A P Jadhav, V J Kadam
Affiliations

A Validated High-performance Liquid Chromatograhy Method for Estimation of Ferulic Acid in Asafoetida and Polyherbal Preparation

J A Kareparamban et al. Indian J Pharm Sci. 2013 Jul.

Abstract

A high-performance liquid chromatography method was developed for the estimation of ferulic acid from asafoetida and a polyherbal preparation. The separation was carried out on HiQSil ODS C-18 column with a mobile phase of acetonitrile: 10% acetic acid (20:80 v/v). The developed method was validated as per International Conference of Harmonization guidelines for various parameters such as accuracy, precision, linearity, limit of detection, limit of quantification and specificity; and found to be reliable. Linear regression analysis showed a good corelation between peak area and concentration with a corelation coefficient r(2)=0.996 in the range 200-7000 ng/ml. The developed method can be utilized for standardization of herbal formulation comprising asafoetida.

Keywords: High-performance liquid chromatograhy; asafoetida; ferulic acid; polyherbal formulations..

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
HPLC chromatograms of standard, asafoetida methanol extract, and polyherbal preparation. Chromatograms showing. (a) ferulic acid (b) methanol extract of asafoetida and (c) polyherbal preparation.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Shah B, Seth A. 1st ed. New York: Elsevier; 2010. Textbook of Pharmacognosy and Phytochemistry; pp. 319–21.
    1. Wallis TE. 5th ed. New York: CBS Publisher; 2004. Textbook of Pharmacognosy; pp. 503–5.
    1. Mohammad F, Freshteh F, Hassanabad ZF. Antispasmodic and hypotensive activity of Ferula asafoetida gum extract. J Ethnopharmacol. 2004;91:321–4. - PubMed
    1. Gholamnezhad Z, Byrami G, Boskabady MH. Possible mechanism of the relaxant effect of asafoetida. Avicenna J Phytomed. 2012;2:10–6.
    1. Houghton PJ, Apendino G, Maxia L. A meroterpenoid NF-ĸβ inhibitor and drimane sesquiterpenoids from asafoetida. J Nat Prod. 2006;69:1101–4. - PubMed

LinkOut - more resources