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. 2013 Jul;75(4):493-5.
doi: 10.4103/0250-474X.119826.

A Validated High-performance Liquid Chromatograhy Method for Estimation of Ferulic Acid in Asafoetida and Polyherbal Preparation

J A Kareparamban  1 , P H Nikam, A P Jadhav, V J Kadam
Affiliations

A Validated High-performance Liquid Chromatograhy Method for Estimation of Ferulic Acid in Asafoetida and Polyherbal Preparation

J A Kareparamban et al. Indian J Pharm Sci. 2013 Jul.

Abstract

A high-performance liquid chromatography method was developed for the estimation of ferulic acid from asafoetida and a polyherbal preparation. The separation was carried out on HiQSil ODS C-18 column with a mobile phase of acetonitrile: 10% acetic acid (20:80 v/v). The developed method was validated as per International Conference of Harmonization guidelines for various parameters such as accuracy, precision, linearity, limit of detection, limit of quantification and specificity; and found to be reliable. Linear regression analysis showed a good corelation between peak area and concentration with a corelation coefficient r(2)=0.996 in the range 200-7000 ng/ml. The developed method can be utilized for standardization of herbal formulation comprising asafoetida.

Keywords: High-performance liquid chromatograhy; asafoetida; ferulic acid; polyherbal formulations..

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Figures

Fig. 1
Fig. 1
HPLC chromatograms of standard, asafoetida methanol extract, and polyherbal preparation. Chromatograms showing. (a) ferulic acid (b) methanol extract of asafoetida and (c) polyherbal preparation.
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