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. 2015 Apr;43(2):106-11.
doi: 10.3109/21691401.2013.864662. Epub 2013 Dec 5.

Differentiation of chicken umbilical cord mesenchymal stem cells into beta-like pancreatic islet cells

Chunyu Bai  1 , Yuhua Gao, Qian Li, Yuan Feng, Yanze Yu, Gentong Meng, Minghai Zhang, Weijun Guan
Affiliations
Free article

Differentiation of chicken umbilical cord mesenchymal stem cells into beta-like pancreatic islet cells

Chunyu Bai et al. Artif Cells Nanomed Biotechnol. 2015 Apr.
Free article

Abstract

In this study, we explored the possibility of using in vitro differentiation to create functional beta-like islet cells from chicken umbilical cord mesenchymal stem cells (UCMSCs). Passaged UCMSCs were induced to differentiate into pancreatic beta-like islet cells. Differentiated cells were observed through dithizone staining, and Pdx1 and insulin expressed in differentiated cells were detected with immunofluorescence. Insulin and C-peptide production from differentiated cells were analyzed using ELISA and western blotting. Differentiated cells were found to not only express Pdx1, insulin, and C-peptide, but also to display a glucose-responsive secretion of these hormones.

Keywords: chicken; islet cells; mesenchymal stem cells; umbilical cord.

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