Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Mar;5(2):69-76.
doi: 10.1007/BF00020088.

Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues

S O Rogers  1 , A J Bendich
Affiliations

Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues

S O Rogers et al. Plant Mol Biol. 1985 Mar.

Abstract

We have developed a DNA extraction procedure for milligram amounts of plant tissue. Yields ranged from 0.3-200 nanograms of DNA per milligram of tissue. The factors affecting yield are discussed. Fresh tissue, as well as herbarium specimens (22-118 years old) and mummified seeds and embryos (500 to greater than 44 600 years old) were used. All tissues attempted (57 types from 29 species) yielded measurable amounts of DNA. In no case tested was inhibition observed for restriction enzymes BamHI or EcoRI.

PubMed Disclaimer

References

    1. Plant Physiol. 1975 Mar;55(3):496-501 - PubMed
    1. Philos Trans R Soc Lond B Biol Sci. 1976 May 27;274(933):227-74 - PubMed
    1. Brookhaven Symp Biol. 1972;23:451-94 - PubMed
    1. Nat New Biol. 1973 Oct 10;245(145):170-2 - PubMed
    1. J Biol Chem. 1977 Nov 25;252(22):8118-25 - PubMed

LinkOut - more resources