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. 1979 May;55(3-4):101-6.
doi: 10.1007/BF00295434.

An effective chemical mutagenesis procedure for Petunia hybrida cell suspension cultures

C M Colijn  1 , A J Kool, H J Nijkamp
Affiliations

An effective chemical mutagenesis procedure for Petunia hybrida cell suspension cultures

C M Colijn et al. Theor Appl Genet. 1979 May.

Abstract

By using mercury(II)-chloride (HgCl2) and Dl-6-fluorotryptophan (6FT) as positive selection conditions we were able to show that N-methyl-N'-nitro-N-nitrosoguanidine (NG) is an effective mutagen for Petunia hybrida suspension cells.A number of the 205 calli resistant to HgCl2 and 17 calli resistant to 6FT were isolated. The highest mutation frequency was 1.0 x 10(-5) and 2.0 x 10(-6) for HgCl2 and 6FT, respectively. A preliminary characterization of the mutants is presented.A significant increase in the number of drug-resistant calli was only obtained at NG-concentrations (5-40 μg/ml) that had no observable effect on the survival of the mutagenized cultures.

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References

    1. Genetics. 1976 Sep;84(1):51-7 - PubMed
    1. Stain Technol. 1972 Jul;47(4):189-94 - PubMed
    1. Biochim Biophys Acta. 1972 Aug 18;279(1):48-57 - PubMed