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. 1979 Nov;55(6):257-62.
doi: 10.1007/BF00265362.

Biochemical selection of immature, haploid embryos of Zea mays L

H S Dhaliwal  1 , P J King
Affiliations

Biochemical selection of immature, haploid embryos of Zea mays L

H S Dhaliwal et al. Theor Appl Genet. 1979 Nov.

Abstract

A method was devised for the biochemical selection of immature, haploid Zea mays embryos using Adh1 (-) and either the Stock 6 or indeterminate gametophyte (ig in W23) high haploid-inducing systems. Haploid (Adh1 (-)) embryos survived exposure to levels of allyl alcohol which killed diploid (Adh1 (+)/Adh1 (-)) embryos. Of the total surviving embryos which were examined cytologically 15% (using ig) and 22% (using Stock 6) were haploid. In two experiments with Stock 6, 100% of the surviving embryos were haploid. To obtain maximum effectiveness of Stock 6 and ig, Adh1 (-) was transferred to stock 6 and W23 backgrounds. Immature, haploid embryos are being used to develop haploid, morphogenic tissue cultures of Zea mays.

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