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. 1985 Sep;5(5):299-302.
doi: 10.1007/BF00020627.

A chimaeric hygromycin resistance gene as a selectable marker in plant cells

P J van den Elzen  1 , J Townsend, K Y Lee, J R Bedbrook
Affiliations

A chimaeric hygromycin resistance gene as a selectable marker in plant cells

P J van den Elzen et al. Plant Mol Biol. 1985 Sep.

Abstract

We show here that plant cells are sensitive to the antibiotic hygromycin-B(4). We also show that a chimaeric gene consisting of the nopaline synthase (nos) gene regulatory elements and the E. coli derived hygromycin phosphotransferase (hpt) gene, when transferred to plants' cells, confers resistance to hygromycin B. The chimaeric nos-hpt gene enables efficient selection of DNA transfer to plant cells when used in conjunction with Ti plasmid-derived binary vectors in cocultivation experiments.

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