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. 1986 Dec 5;47(5):649-56.
doi: 10.1016/0092-8674(86)90507-6.

Transfection of a DNA locus that mediates the conversion of 10T1/2 fibroblasts to myoblasts

A B Lassar, B M Paterson, H Weintraub

Transfection of a DNA locus that mediates the conversion of 10T1/2 fibroblasts to myoblasts

A B Lassar et al. Cell. .

Abstract

Stable myoblast cell lines were isolated after a brief exposure of mouse fibroblasts (10T1/2 cells) to 5-azacytidine. We show that transfection of 10T1/2 cells with DNA from these azacytidine-induced myoblasts (or from mouse C2C12 myoblasts) results in myogenic conversion of approximately 1 in 15,000 transfected colonies. In contrast, transfection of 10T1/2 cells with DNA from nonmyogenic cells (parental 10T1/2 cell DNA) does not give rise to myoblast colonies. These results indicate that an azacytidine-induced structural modification (presumably demethylation) in the DNA of a single locus is sufficient to convert 10T1/2 cells into determined myoblasts.

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  • From MYOD1 to iPS cells.
    Hochedlinger K. Hochedlinger K. Nat Rev Mol Cell Biol. 2010 Dec;11(12):817. doi: 10.1038/nrm3018. Nat Rev Mol Cell Biol. 2010. PMID: 21102604 No abstract available.

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