Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Jan;3(1):37-44.
doi: 10.1007/BF00023414.

Localization of the replicase recognition site within brome mosaic virus RNA by hybrid-arrested RNA synthesis

P Ahlquist  1 , J J Bujarski, P Kaesberg, T C Hall
Affiliations

Localization of the replicase recognition site within brome mosaic virus RNA by hybrid-arrested RNA synthesis

P Ahlquist et al. Plant Mol Biol. 1984 Jan.

Abstract

3' terminal fragments of BMV RNA as short as 153 bases in length serve as efficient templates in vitro for BMV-specific RNA polymerase. Template activity of such fragments or of native BMV RNA is abolished when cDNA fragments as short as 39 bases are hybridized to their 3' termini. Hybridization of cDNa fragments to regions of BMV RNA 200 or more bases distal to the 3' end has no discernible effect on initiation and little effect on elongation. We conclude that BMV RNA polymerase initiates binding with an RNA template through a mechanism mediated by the tRNA-like 3' end of BMV RNA, requiring at least some of the last 39, but no more than the last 153 bases.

PubMed Disclaimer

References

    1. Nature. 1976 Sep 9;263(5573):106-9 - PubMed
    1. J Virol. 1982 Oct;44(1):209-16 - PubMed
    1. Nucleic Acids Res. 1981 May 25;9(10):2251-66 - PubMed
    1. Nucleic Acids Res. 1982 Jan 22;10(2):703-13 - PubMed
    1. J Mol Biol. 1980 Oct 25;143(2):161-78 - PubMed

LinkOut - more resources