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. 1984 Jul;3(4):235-41.
doi: 10.1007/BF00029659.

Cloning of Petunia hybrida chloroplast DNA sequences capable of autonomous replication in yeast

N Overbeeke  1 , M A Haring, H John, J Nijkamp, A J Kool
Affiliations

Cloning of Petunia hybrida chloroplast DNA sequences capable of autonomous replication in yeast

N Overbeeke et al. Plant Mol Biol. 1984 Jul.

Abstract

Sequences from Petunia hybrida chloroplast DNA which have the property to promote autonomous replication in Saccharomyces cerevisiae were cloned in vector YIp5. Seven cloned chloroplast DNA fragments are localized at one of two different sites on the chloroplast genome. One site, arsA was mapped on a 1.8 Kb fragment at position 27.0-28.8 Kb on the P. hybrida chloroplast genome. The plasmids containing this arsA are stable both in yeast and E. coli. The other site, arsB, was shown to be very unstable and is located either in the small single copy region close to the inverted repeat or just in the inverted repeat. The functioning of these sequences as a possible origin of replication in vivo is discussed.

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References

    1. J Bacteriol. 1983 Jun;154(3):1033-9 - PubMed
    1. Gene. 1980 Oct;11(1-2):11-9 - PubMed
    1. Adv Exp Med Biol. 1984;179:107-12 - PubMed
    1. J Mol Appl Genet. 1982;1(4):327-41 - PubMed
    1. EMBO J. 1983;2(10):1765-70 - PubMed

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