Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Nov;3(6):445-52.
doi: 10.1007/BF00033392.

Microanalysis of plant mitochondrial protein synthesis products: : Detection of variant polypeptides associated with cytoplasmic male sterility

M Boutry  1 , A M Faber, M Charbonnier, M Briquet
Affiliations

Microanalysis of plant mitochondrial protein synthesis products: : Detection of variant polypeptides associated with cytoplasmic male sterility

M Boutry et al. Plant Mol Biol. 1984 Nov.

Abstract

A mitochondrial fraction obtained from 0.5 g of leaves was purified on a 0.75 ml Percoll gradient and used for an in vitro mitochondrial protein synthesis assay in the presence of [(35)S] methionine. A set of 15 to 20 labeled polypeptides were revealed by autoradiography after sodium dodecylsulfate polyacrylamide gel electrophoresis. This could be applied at an early growth stage by using a few leaves from individual seedlings. It revealed the presence of variant mitochondrially translated polypeptides in green leaves of cytoplasmic male sterile lines from various cultivated plants of large economic importance: maize, wheat, sugar beet, tobacco and faba bean. This non-destructive microanalysis is thus of general use and opens new possibilities for rapid and large mass screening of mitochondrial parameters such as male sterility.

PubMed Disclaimer

References

    1. J Biol Chem. 1983 Jul 25;258(14):8524-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3841-5 - PubMed
    1. Plant Mol Biol. 1982 Jun;1(2):89-102 - PubMed
    1. EMBO J. 1983;2(10):1783-9 - PubMed
    1. Mol Gen Genet. 1982;186(1):50-6 - PubMed