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. 1985 Jan;4(1):55-60.
doi: 10.1007/BF02498715.

The detection of PR (b) protein and TMV by ELISA in systemic and localised virus infections of tobacco

J F Antoniw  1 , R F White, D J Barbara, P Jones, A Longley
Affiliations

The detection of PR (b) protein and TMV by ELISA in systemic and localised virus infections of tobacco

J F Antoniw et al. Plant Mol Biol. 1985 Jan.

Abstract

An antiserum was prepared to the b1 protein purified from TMV infectedN. tabacum cv. Xanthi-nc leaves and used to study PR proteins. The Xanthi-nc proteins b2 and b3 were shown to be serologically closely related to b1. Antisera to b1 protein and TMV were used in a F(ab')2 enzyme linked immunosorbent assay to monitor PR protein and TMV concentrations, respectively, during the first 6 days of a systemic TMV infection (cv. Xanthi) and a localised TMV infection (cv. Xanthi-nc).

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