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. 1985 Jul;4(4):205-10.
doi: 10.1007/BF02418237.

Characterization of developing oat seed mRNA: evidence for many globulin mRNAs

S Fabijanski  1 , G J Matlashewski, I Altosaar
Affiliations

Characterization of developing oat seed mRNA: evidence for many globulin mRNAs

S Fabijanski et al. Plant Mol Biol. 1985 Jul.

Abstract

Polyadenylated mRNA from developing oat (Avena sativa L.) seeds was isolated and analyzed. Prominent mRNA species of 18S, 15S and 12S were observed; the 18S mRNA was judged to be esentially free of ribosomal RNA by hybridization analysis. Size fractionation andin vitro translation of this mRNA was performed. SDS, IEF-SDS gel electrophoresis and immunoprecipitation were used to analyze the translation products. It is shown that globulin mRNA (18S) accounts for roughly 30% of the total mRNA in developing seeds, the 12S and 15S mRNAs accounting for the remainder. The 18S mRNA directs the synthesis of a series of distinct but related polypeptides, suggesting that some of the heterogeneity seen in the oat globulins is at the amino acid sequence level.

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