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. 2013;3(1):103-8.
doi: 10.5681/apb.2013.017. Epub 2013 Feb 7.

Phytochemical Screening and Anti-nociceptive Properties of the Ethanolic Leaf Extract of Trema Cannabina Lour

Hemayet Hossain  1 , Ismet Ara Jahan, Howlader Sariful Islam, Dey Shubhra Kanti, Hira Arpona, Ahmed Arif
Affiliations

Phytochemical Screening and Anti-nociceptive Properties of the Ethanolic Leaf Extract of Trema Cannabina Lour

Hemayet Hossain et al. Adv Pharm Bull. 2013.

Abstract

Purpose: The present study was designed to investigate the anti-nociceptive activity of ethanolic leaf extract of Trema cannabina Lour (family: Cannabaceae) in experimental animal models.

Methods: The anti-nociceptive action was carried out against two types of noxious stimuli, thermal (hot plate and tail immersion tests) and chemical (acetic acid-induced writhing) in mice.

Results: Phytochemical analysis of crude extract indicated the presence of reducing sugar, tannins, steroid and alkaloid types of secondary metabolites. Crude extract of T. cannabina (500 mg/kg dose) showed maximum time needed for the response against thermal stimuli (6.79±0.15 seconds) which is comparable to diclofenac sodium (8.26±0.14 seconds) in the hot plate test. Hot tail immersion test also showed similar results as in hot plate test. At the dose of 250 and 500 mg/kg body weight, the extract showed significantly and in a dose-dependent (p<0.001) reduction in acetic acid induced writhing in mice with a maximum effect of 47.56% reduction at 500 mg/kg dose comparable to that of diclofenac sodium (67.07%) at 25 mg/kg.

Conclusion: The obtained results tend to suggest the Anti-nociceptive activity of ethanolic leaf extract of Trema cannabina and thus provide the scientific basis for the traditional uses of this plant part as a remedy for pain.

Keywords: Acetic acid induced writhing test; Anti-nociceptive; Hot plate test; Tail immersion test; Trema cannabina.

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References

    1. Uddin SN, Uddin KMA, Ahmed F. Analgesic and antidiarrhoeal activities of Treamaorientalis Linnin mice. Oriental Pharm and Exp Med . 2008;8(2):187–91.
    1. Kirtikar KR, Basu BD. Indian Med Plants. 2nd ed. India: Singh B and Singh MP publishers; 1980.
    1. Iwe MM. Handbook of african medicinal plants. Boca Raton: CRC Press; 1993.
    1. Rulangaranga ZK. Conservation of medicinal and aromatic plants in Tanzania proceedings of a workshop on priority species for tree planting and afforestation in Tanzania. Tanzania: Morogoro; 1991.
    1. Ghani A. Medicinal Plants of Bangladesh. 1st ed. Bangladesh: Dhaka; 1998.

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