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Case Reports
. 2013 Dec 5:13:282.
doi: 10.1186/1471-2180-13-282.

First identification of coexistence of blaNDM-1 and blaCMY-42 among Escherichia coli ST167 clinical isolates

Xueqing Zhang, Danping Lou, Yuanyuan Xu, Yongpeng Shang, Dan Li, Xiaoying Huang, Yuping Li, Longhua Hu, Liangxing Wang  1 , Fangyou Yu
Affiliations
Case Reports

First identification of coexistence of blaNDM-1 and blaCMY-42 among Escherichia coli ST167 clinical isolates

Xueqing Zhang et al. BMC Microbiol. .

Abstract

Background: Emergence of multidrug resistance in Enterobacteriaceae limits the selection of antimicrobials for treatment of infectious diseases. Identification of NDM-1 makes more difficulty in treating multidrug-resistant Enterobacteriaceae infections. Carbapenem-resistant Escherichia coli clinical isolates from a tertiary hospital in Wenzhou, east China, were investigated for NDM-1 production.

Results: The two tested isolates were negative for modified Hodge test, but positive for a double-disc synergy test used for detecting metallo-β-lactamase production. E. coli WZ33 and WZ51 exhibited discrepant-level resistance to most clinically frequent used antimicrobials, but still susceptible to trimethoprim/sulfamethoxazole, amikacin, fosfomycin, tigecycline and polymyxin B. E. coli WZ33 and WZ51 were positive for bla(NDM-1) determined by PCR and DNA sequencing. Other than bla(NDM-1), E. coli WZ33 also harbored bla(CTX-M-14) and bla(CMY-42), while E. coli WZ51 simultaneously harbored blaSHV-12,bla(CTX-M-14) and bla(CMY-42). Carbapenem resistance for E. coli WZ51 and WZ33 could not be transferred to E. coli recipients through conjugation, but could be transferred to E. coli recipients by chemical transformation. The EcoR1-digested DNA pattern of plasmids from the transformant of E. coli WZ51 was different from that of E. coli WZ51. MLST showed that E. coli WZ33 and WZ51 belonged to an animal-associated clone (ST167).

Conclusion: The present study is the first report of bla(NDM-1) carriage in E. coli ST167 isolates and coexistence of bla(NDM-1) and bla(CMY-42) in same isolate. Systemic surveillance should focus on the dissemination of bla(NDM-1) among Enterobacteriaceae, especially E. coli ST167 clone associated with animal infection.

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Figures

Figure 1
Figure 1
EcoR1-digested DNA patterns of plasmids from the transformants of E. coli WZ33 and WZ51. M, the DNA ladder; A, Digest of plasmid from the transformant of E.coli WZ33; B, Digest of plasmid from the transformant of E.coli WZ51.
See this image and copyright information in PMC

References

    1. Tzouvelekis LS, Markogiannakis A, Psichogiou M, Tassios PT, Daikos GL. Carbapenemases in Klebsiella pneumoniae and other Enterobacteriaceae: an evolving crisis of global dimensions. Clin Microbiol Rev. 2012;13(4):682–707. doi: 10.1128/CMR.05035-11. - DOI - PMC - PubMed
    1. Rapp RP, Urban C. Klebsiella pneumoniae carbapenemases in Enterobacteriaceae: history, evolution, and microbiology concerns. Pharmacotherapy. 2012;13(5):399–407. doi: 10.1002/j.1875-9114.2012.01035.x. - DOI - PubMed
    1. Maltezou HC. Metallo-beta-lactamases in gram-negative bacteria: introducing the era of pan-resistance? Int J Antimicrob Agents. 2009;13(5):405. e401-407. - PubMed
    1. Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, Lee K, Walsh TR. Characterization of a new metallo-beta-lactamase gene, bla (NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob Agents Chemother. 2009;13(12):5046–5054. doi: 10.1128/AAC.00774-09. - DOI - PMC - PubMed
    1. Kumarasamy KK, Toleman MA, Walsh TR, Bagaria J, Butt F, Balakrishnan R, Chaudhary U, Doumith M, Giske CG, Irfan S. et al.Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect Dis. 2010;13(9):597–602. doi: 10.1016/S1473-3099(10)70143-2. - DOI - PMC - PubMed

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