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. 2014 Mar;133(3):910-4.e5.
doi: 10.1016/j.jaci.2013.10.004. Epub 2013 Dec 6.

Allergen microarray detects high prevalence of asymptomatic IgE sensitizations to tropical pollen-derived carbohydrates

Clarissa R Cabauatan  1 , Christian Lupinek  2 , Sandra Scheiblhofer  3 , Richard Weiss  3 , Margarete Focke-Tejkl  4 , Prem L Bhalla  5 , Mohan B Singh  5 , Paul A Knight  5 , Marianne van Hage  6 , John Donnie A Ramos  7 , Rudolf Valenta  8
Affiliations

Allergen microarray detects high prevalence of asymptomatic IgE sensitizations to tropical pollen-derived carbohydrates

Clarissa R Cabauatan et al. J Allergy Clin Immunol. 2014 Mar.
No abstract available

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Conflict of interest statement

Disclosure of potential conflict of interest: C. Lupinek has received one or more payments for lecturing from or is on the speakers’ bureau for Thermo Fisher Scientific. M. van Hage has received one or more payments for lecturing from or is on the speakers’ bureau for Thermo Fisher Scientific, Novartis, and ALK-Abell'o. R. Valenta has been supported by one or more grants from the Austria Science Fund, Biomay AG, and Thermo Fisher Scientific, and has received one or more consulting fees or honorarium from Biomag AG and Thermo Fisher Scientific. The rest of the authors declare that they have no relevant conflicts of interest.

Figures

Fig E1
Fig E1
Presence of allergens in timothy grass and tropical grasses. Pollen extracts were subjected to SDS-PAGE and silver stained (lanes a), and after Western blotting, analyzed by using rabbit antisera specific for grass pollen allergen groups (lanes a group 1, 2, 5, 6, 7, and 13), normal rabbit antibodies (lanes nra), or buffer (lanes b). Molecular weights are indicated on the left margins.
Fig E2
Fig E2
IgE reactivity of individual sera (y-axis: ISU) to allergens on ImmunoCAP ISAC after periodate treatment (deglycosylated ISAC) or after exposure to buffer alone (untreated ISAC).
Fig E3
Fig E3
Quantitative IgE binding (y-axis: kUA/L) of 11 nPhl p 4-positive symptomatic, 3 asymptomatic subjects, and 4 α-Gal–positive sera (positive controls) (x-axis) to α-Gal.
Fig E4
Fig E4
Induction of rat basophil leukemia cell degranulation (y-axis) with different concentrations of timothy grass pollen allergens (x-axis) when cells were loaded with serum IgE from a patient allergic to timothy grass pollen versus lack of degranulation when serum from a nonallergic individals or buffer was used.
Fig 1
Fig 1
IgE reactivities of symptomatic and asymptomatic individuals from the Philippines to micro-arrayed allergens. A, IgE reactivities (y-axes: ISU) of symptomatic (dots) and asymptomatic individuals (triangles) to allergens on the chip (x-axes: allergens, allergen sources, number of positive sera, allergens with known glycosylation are underlined). B, Summary of the frequencies of IgE reactivities in symptomatic and asymptomatic individuals according to allergen sources.
Fig 2
Fig 2
A, IgE reactivity (y-axis) of sera from subjects with grass pollen–specific IgE to nCyn d 1 (left panel) and nPhl p 4 (right panel) after preincubation with (+) or without (−) nPhl p 4. B, IgE reactivity (y-axis) of sera to untreated (−) and deglycosylated (+) nCyn d 1 and nPhl p 4. C, Mediator release induced by grass pollen allergens (x-axis) displayed as percentages of total β-hexosaminidase (y-axes) for symptomatic and asymptomatic subjects or buffer.
See this image and copyright information in PMC

References

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