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. 1979 Jan;145(4):371-5.
doi: 10.1007/BF00388363.

Selection of somatic hybrids after fusion of protoplasts from Datura innoxia Mill. and Atropa belladonna L

G Krumbiegel  1 , O Schieder
Affiliations

Selection of somatic hybrids after fusion of protoplasts from Datura innoxia Mill. and Atropa belladonna L

G Krumbiegel et al. Planta. 1979 Jan.

Abstract

After fusion of protoplasts from a diploid (2n=24) and a tetraploid (4n=48) chlorophyll-deficient mutant of Datura innoxia Mill. with diploid (2n=72) green wild-type protoplasts of Atropa belladonna L. thirteen somatic hybrids could be selected, most of which had already started to produce leaves and shoots. Hybrid calli were recognizable by the production of hairs, typical for Datura innoxia, and the green colour, derived from Atropa belladonna. Further proof for the hybrid nature was furnished by cytological investigations. The metaphase chromosomes of both species are easily distinguishable in their size: chromosomes of Datura innoxia are about twice as large as those of Atropa belladonna. The chromosome numbers of the hybrids varied from ca. 84 to ca. 175.

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