Biosynthesis and structure of stable branched RNA covalently linked to the 5' end of multicopy single-stranded DNA of Stigmatella aurantiaca

Abstract

Stigmatella aurantiaca, a gram-negative bacterium, contains approximately 500 copies per cell of a short single-stranded linear DNA (multicopy single-stranded DNA: msDNA). This DNA is attached to a branched RNA (msdRNA) by its 5' end. The entire sequence of msdRNA was determined and found to consist of 76 bases. The msDNA is linked at the 19th G residue of msdRNA by a 2', 5' phosphodiester linkage. The coding region for msdRNA (msr) is located downstream of the coding region for msDNA (msd). These coding regions exist in opposite orientation with respect to each other and overlap by 8 bases at their 3' ends. Biosynthesis of RNA-linked msDNA was characterized and mechanisms of synthesis are proposed.