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. 2014 Feb;196(2):397-401.
doi: 10.1534/genetics.113.160135. Epub 2013 Dec 6.

Three potato centromeres are associated with distinct haplotypes with or without megabase-sized satellite repeat arrays

Affiliations

Three potato centromeres are associated with distinct haplotypes with or without megabase-sized satellite repeat arrays

Linsheng Wang et al. Genetics. 2014 Feb.

Abstract

We report discoveries of different haplotypes associated with the centromeres of three potato chromosomes, including haplotypes composed of long arrays of satellite repeats and haplotypes lacking the same repeats. These results are in favor of the hypothesis that satellite repeat-based centromeres may originate from neocentromeres that lack repeats.

Keywords: Centromere; centromere evolution; neocentromere; placeholder; satellite repeat.

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Figures

Figure 1
Figure 1
FISH mapping of the Cen2-specific satellite repeat St3.58 in four potato cultivars. Potato chromosome 2 is identified by the 45S rDNA probe (red signals), which is located on the short arm of chromosome 2. (A) FISH signal from St3.58 (arrow) was detected on a single Cen2 in Atlantic. (A′) The FISH signal from St3.58 was digitally separated from A. (B) FISH signals from St3.58 (arrows) were detected on two of the four copies of Cen2 in Katahdin. (B′) The FISH signals from St3.58 were digitally separated from B. (C) FISH signals from St3.58 (arrows) were detected on all four copies of Cen2 in Atzimba. (C′) The FISH signals from St3.58 were digitally separated from C. (D) St3.58 did not hybridize to any of the four copies of Cen2 in White Pearl. The two arrowheads point to fused 45S rDNA signals from two chromosomes. (D′) The FISH signals from St3.58 were digitally separated from D. Plasmid 1331 was used to detect the St. 3.58 repeat. Bars, 5 µm.
Figure 2
Figure 2
Southern blot hybridization analysis of the centromeric satellite repeats in different potato cultivars. (A) Southern blot hybridization analysis of the Cen2-specific satellite repeat St3.58 in four potato cultivars. The numbers in the parentheses indicate the copy number of chromosome 2 carrying St3.58 in each cultivar. Left: genomic DNAs from the four cultivars were digested by BamHI and separated by a 0.8% agarose gel. Right: blotted DNAs were hybridized to St3.58 (plasmid probe 1331). No hybridization was found to DNA of White Pearl. (B) Southern blot hybridization analysis of the Cen1-specific satellite repeat St24 in four potato cultivars. The numbers in parentheses indicate the copy number of chromosome 2 carrying St24 in each cultivar. Left: genomic DNAs from the four cultivars were digested by HindIII and separated by a 0.8% agarose gel. Right: blotted DNAs were hybridized to St24 (plasmid probe 1336). No hybridization was found to DNA of Superior. Southern blot hybridization followed published protocol (Stupar et al. 2002).
Figure 3
Figure 3
FISH mapping of the satellite repeats St24 and St57 in tetraploid potato genotypes. (A–D) FISH mapping of the Cen1-specific satellite repeat St24 in four potato cultivars, Hindenburg, Kennebec, Juanita, and Superior, respectively. Potato chromosome 1 was identified by bacterial artificial chromosome clone 96H03 (red signals). Arrows point to strong FISH signals and arrowheads point to weak FISH signals. (A′–D′) The FISH signals from St24 were digitally separated from A to D, respectively. (E–H) FISH mapping of the Cen7-specific satellite repeat St57 in four potato cultivars, White Pearl, MegaChip, Kerr’s Pink, and Katahdin, respectively. Potato chromosome 7 was identified by bacterial artificial chromosome clone 186I02 (red signals). Arrows point to strong FISH signals and arrowheads point to weak FISH signals. (E′–H′) The FISH signals from St57 were digitally separated from E to H, respectively. Plasmids 1336 and 1338 were used to detect repeats St24 and St57, respectively. Bars, 5 µm.

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