Plastid thioredoxins: a "one-for-all" redox-signaling system in plants

Abstract

The sessile nature of plants forces them to face an ever-changing environment instead of escape from hostile conditions as animals do. In order to overcome this survival challenge, a fine monitoring and controlling of the status of the photosynthetic electron transport chain and the general metabolism is vital for these organisms. Frequently, evolutionary plant adaptation has consisted in the appearance of multigenic families, comprising an array of enzymes, structural components, or sensing, and signaling elements, in numerous occasions with highly conserved primary sequences that sometimes make it difficult to discern between redundancy and specificity among the members of a same family. However, all this gene diversity is aimed to sort environment-derived plant signals to efficiently channel the external incoming information inducing a right physiological answer. Oxygenic photosynthesis is a powerful source of reactive oxygen species (ROS), molecules with a dual oxidative/signaling nature. In response to ROS, one of the most frequent post-translational modifications occurring in redox signaling proteins is the formation of disulfide bridges (from Cys oxidation). This review is focused on the role of plastid thioredoxins (pTRXs), proteins containing two Cys in their active site and largely known as part of the plant redox-signaling network. Several pTRXs types have been described so far, namely, TRX f, m, x, y, and z. In recent years, improvements in proteomic techniques and the study of loss-of-function mutants have enabled us to grasp the importance of TRXs for the plastid physiology. We will analyze the specific signaling function of each TRX type and discuss about the emerging role in non-photosynthetic plastids of these redox switchers.

Keywords: carbon metabolism; oxidative stress; photosynthesis; redox signaling; thioredoxin.

FIGURE 1

Scheme of the TRX f and m-mediated redox signaling in plastids. (A) Main physiological processes related to photosynthesis and retrograde signaling controlled by TRX f and/or m are shown in the figure. (B) Regulation of carbon metabolism carried out by TRX f and/or m.

FIGURE 2

Scheme of TRX x, y, and z-mediated redox signaling in plastids. The central physiological processes regulated by these isoforms are shown.

FIGURE 3

Scheme of the pTRXs implication in the chloroplast signaling network.