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. 2014 Mar:197:25-8.
doi: 10.1016/j.jviromet.2013.11.017. Epub 2013 Dec 7.

Identification of vaccine-derived polioviruses using dual-stage real-time RT-PCR

David R Kilpatrick  1 , Karen Ching  2 , Jane Iber  2 , Qi Chen  2 , Su-Ju Yang  2 , Lina De  2 , A J Williams  2 , Mark Mandelbaum  2 , Hong Sun  2 , M Steven Oberste  2 , Olen M Kew  2
Affiliations

Identification of vaccine-derived polioviruses using dual-stage real-time RT-PCR

David R Kilpatrick et al. J Virol Methods. 2014 Mar.

Abstract

Vaccine-derived polioviruses (VDPVs) are associated with polio outbreaks and prolonged infections in individuals with primary immunodeficiencies. VDPV-specific PCR assays for each of the three Sabin oral poliovirus vaccine (OPV) strains were developed, targeting sequences within the VP1 capsid region that are selected for during replication of OPV in the human intestine. Over 2400 Sabin-related isolates and identified 755 VDPVs were screened. Sensitivity of all assays was 100%, while specificity was 100% for serotypes 1 and 3, and 76% for serotype 2. The assays permit rapid, sensitive identification of OPV-related viruses and flag programmatically important isolates for further characterization by genomic sequencing.

Keywords: Poliovirus; Real-time RT-PCR; VDPV; Vaccine-derived poliovirus.

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Figures

Fig. 1.
Fig. 1.
Frequency of changes at each VP1 amino acid position for 34 VDPV1s (A), 702 VDPV2s (B), and 19 VDPV3s (C). The TaqMan probes targeted the identified amino acid as well as amino acids either upstream or downstream to the primary target, depending on the serotype.
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