The effects of rubidium ions on components of the potassium conductance in the frog node of Ranvier

Abstract

The effects of replacement of external and internal K+ ions by Rb+ ions on the two fast components (gf1 and gf2) and slow component (gs) of the K+ conductance (gK) in frog nodes of Ranvier were investigated under voltage- and current-clamp conditions. Fast and slow components of gK were separated by double exponential fits to tail currents following long depolarizing pre-pulses, or by the use of short pre-pulses which activate little gs X gs was also isolated by 1 mM-4-aminopyridine (4-AP). gf1 and gf2 were distinguished in the fast conductance-voltage curve by their different voltage dependences, gf1 activating at more negative potentials. Reversal potential measurements indicated that Rb+ is less permeant than K+, and measurements in 4-AP indicated that the slow component has a lower Rb+ permeability than the fast. In a 50% K+, 50% Rb+ mixture PRb/PK was less than that in 100% Rb+ suggesting that PRb/PK is mole-fraction dependent. With external Rb+ the current-voltage relation was shifted by ca.-10 mV compared to that in K+, an effect on gf ( = gf1 + gf2). The slow conductance (gs) and, under similar conditions, the Na+ current-voltage relation were not shifted. gf, calculated from inward tail currents, was reduced with external Rb+ at potentials where gf2 was activated. Instantaneous current-voltage relations following pre-pulses which activate different components of gf confirmed these observations. In K+ the instantaneous current-voltage relation showed some inward rectification which was largely abolished with Rb+. Comparison of gf calculated from outward (go) and inward (gi) currents confirmed this, and showed that inward gf2 was reduced with Rb+ such that go = gi. Outward currents were little affected by external Rb+. External Rb+ slowed the fast inward tail current following all pre-pulses which activate gf, but had no effect on the time course of the slow component of the tail current. Regenerative responses, which occur in high [K+] (+300 nM-tetrodotoxin) solutions in current clamp did not repolarize in Rb+. Voltage-clamp experiments showed that inactivation of inward currents is slowed when Rb+ is the charge carrier. Replacement of internal K+, by application of Rb+ to the cut ends of the fibre, shifted the reversal potential to more positive potentials but had no effect on the conductance or kinetics. External Rb+ has a large number of effects on inward currents, but little effect on outward currents. Internal Rb+ had little effect on outward or inward currents.(ABSTRACT TRUNCATED AT 400 WORDS)