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. 2013 Dec 6;8(12):e81152.
doi: 10.1371/journal.pone.0081152. eCollection 2013.

Biotyping and genotyping (MLVA16) of Brucella abortus isolated from cattle in Brazil, 1977 to 2008

Sílvia Minharro  1 , Juliana P Silva Mol, Elaine M S Dorneles, Rebeca B Pauletti, Heinrich Neubauer, Falk Melzer, Fernando P Poester, Maurício G Dasso, Elaine S Pinheiro, Paulo M Soares Filho, Renato L Santos, Marcos B Heinemann, Andrey P Lage
Affiliations

Biotyping and genotyping (MLVA16) of Brucella abortus isolated from cattle in Brazil, 1977 to 2008

Sílvia Minharro et al. PLoS One. .

Abstract

Brucellosis is a worldwide distributed zoonosis that causes important economic losses to animal production. In Brazil, information on the distribution of biovars and genotypes of Brucella spp. is scarce or unavailable. This study aimed (i) to biotype and genotype 137 Brazilian cattle isolates (from 1977 to 2008) of B. abortus and (ii) to analyze their distribution. B. abortus biovars 1, 2 and 3 (subgroup 3b) were confirmed and biovars 4 and 6 were first described in Brazil. Genotyping by the panel 1 revealed two groups, one clustering around genotype 40 and another around genotype 28. Panels 2A and 2B disclosed a high diversity among Brazilian B. abortus strains. Eighty-nine genotypes were found by MLVA16. MLVA16 panel 1 and 2 showed geographic clustering of some genotypes. Biotyping and MLVA16 genotyping of Brazilian B. abortus isolates were useful to better understand the epidemiology of bovine brucellosis in the region.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Sampling and distribution of biovars and MLVA16 Panel 2 genotypes of B. abortus isolates from Brazil, 1977 to 2008.
Figure 2
Figure 2. Minimum Spanning Tree (MST) analysis of B. abortus isolates using the MLVA16 data.
The minimum spanning tree presented is the one with the highest overall reliability score and was calculated using UPGMA associated with the priority rule and the bootstrap resampling for strains of Brucella abortus isolated from cattle in Brazil, 1977 to 2008, the reference strains for each biovar of B. abortus and the vaccine strains S19 and RB51. Numbers in each clonal complex represent the genotype on Panel 1. Information on the origin of the isolates was color labeled.
See this image and copyright information in PMC

References

    1. Corbel MJ, Elberg SS, Cosivi O (2006) Brucellosis in humans and animals. Geneva: WHO Press. 89p.
    1. Whatmore AM (2009) Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens. Infect Genet Evol 9: 1168–1184. - PubMed
    1. Poester FP, Gonçalves VSP, Lage AP (2002) Brucellosis in Brazil. Vet Microbiol 90: 55–62. - PubMed
    1. Lucero NE, Ayala SM, Escobar GI, Jacob NR (2008) Brucella isolated in humans and animals in Latin America from 1968 to 2006. Epidemiol Infect 136: 496–503. - PMC - PubMed
    1. Hirsh DC, Zee YC (2003) Microbiologia Veterinária. Rio de Janeiro: Guanabara Koogan. p. 191–195.

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