doi: 10.1016/0378-1119(86)90413-0.
RNA colony hybridization method
- PMID: 2433191
- DOI: 10.1016/0378-1119(86)90413-0
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RNA colony hybridization method
Gene.
1986.
Abstract
A method for rapid screening of specific RNA sequences in recombinant colonies by hybridization in situ is presented. The method includes two consecutive steps of lytic treatment of the nitrocellulose-filter-supported colonies (10% sodium dodecyl sulfate and 3 X SSC at 65 degrees C) and hybridization with 32P-labelled specific oligodeoxynucleotides.
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