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. 1986;46(2-3):287-90.
doi: 10.1016/0378-1119(86)90413-0.

RNA colony hybridization method

I Ivanov, L Gigova

RNA colony hybridization method

I Ivanov et al. Gene. 1986.

Abstract

A method for rapid screening of specific RNA sequences in recombinant colonies by hybridization in situ is presented. The method includes two consecutive steps of lytic treatment of the nitrocellulose-filter-supported colonies (10% sodium dodecyl sulfate and 3 X SSC at 65 degrees C) and hybridization with 32P-labelled specific oligodeoxynucleotides.

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