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. 2014 Apr;134(4):1131-1133.
doi: 10.1038/jid.2013.468. Epub 2013 Nov 11.

p16INK4A influences the aging phenotype in the living skin equivalent

Jean Adamus  1 , Sirpa Aho  2 , Helen Meldrum  2 , Carol Bosko  2 , Jian-Ming Lee  3
Affiliations
Free PMC article

p16INK4A influences the aging phenotype in the living skin equivalent

Jean Adamus et al. J Invest Dermatol. 2014 Apr.
Free PMC article
No abstract available

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Figures

Figure 1
Figure 1
p16INK4A is associated with the aging epidermis. (a) Formalin-fixed, paraffin-embedded (FFPE) sections from young and aged donor keratinocyte living skin equivalent (LSE) models harvested at 7 days post air exposure. Sections were stained with hematoxylin and eosin or immunostained for detection of filaggrin (FLG), Ki-67, and p16INK4A. (b) Expression of p16INK4A protein from monolayer cell lysates (15 μg) harvested at the time of application to the LSE. (c) Representative immunoblot analysis for p16INK4A from young and aged LSE lysates (15 μg) from duplicate cultures harvested at three different time points post air exposure. Bar=100 μm, applies to all FFPE images.
Figure 2
Figure 2
Reversal of phenotype by targeting p16INK4A in the LSE. (a) Young donor keratinocyte living skin equivalents (LSEs) infected with K14 promoter driven either p16INK4A (left) or lacZ control (right) lentivirus, harvested at 11 days post air exposure. Formalin-fixed, paraffin-embedded (FFPE) sections were stained with hematoxylin and eosin (H&E) or immunostained for V5, filaggrin (FLG), Ki-67, or p16INK4A. (b) Immunoblot analysis for caspase-14 (6 μg, left) and for p16INK4A (25 μg, right, bottom). (c) Aged donor keratinocyte LSEs infected with either p16 miR (left) or non-silencing control miR (right) lentivirus, harvested 7 days post air exposure. FFPE sections were stained with H&E or immunostained for filaggrin (FLG), Ki-67, or p16INK4A. (d) LSE lysates (15 μg) were analyzed for p16INK4A and for the differentiation markers loricrin and caspase-14. r-p16, recombinant p16INK4A; e-p16, endogenous p16. Bar=100 μm.
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