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. 2014 May;24(3):709-11.
doi: 10.1007/s10895-013-1341-4. Epub 2013 Dec 13.

Laser induced fluorescence of biochemical for UV LIDAR application

L Gupta  1 , R C Sharma, A K Razdan, A K Maini
Affiliations

Laser induced fluorescence of biochemical for UV LIDAR application

L Gupta et al. J Fluoresc. 2014 May.

Abstract

Laser induced fluorescence spectroscopy in the ultraviolet regime has been used for the detection of biochemical through a fiber coupled CCD detector from a distance of 2 m. The effect of concentration and laser excitation energy on the fluorescence spectra of nicotinamide adenine dinucleotide (NADH) has been investigated. The signature fluorescence peak of NADH was centred about 460 nm. At lower concentration Raman peak centred at 405 nm was also observed. The origin of this peak has been discussed. Detection limit with the proposed set up is found to be 1 ppm.

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