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. 2014 Jan;24(1):130-3.
doi: 10.1038/cr.2013.167. Epub 2013 Dec 17.

A DELLA protein complex controls the arbuscular mycorrhizal symbiosis in plants

Nan Yu  1 , Dexian Luo  1 , Xiaowei Zhang  1 , Junzhong Liu  1 , Wanxiao Wang  1 , Yue Jin  1 , Wentao Dong  1 , Jiyun Liu  1 , Huan Liu  1 , Weibing Yang  1 , Longjun Zeng  1 , Qun Li  1 , Zuhua He  1 , Giles E D Oldroyd  2 , Ertao Wang  1
Affiliations

A DELLA protein complex controls the arbuscular mycorrhizal symbiosis in plants

Nan Yu et al. Cell Res. 2014 Jan.
No abstract available

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Figures

Figure 1
Figure 1
DELLA protein complex is required for the mycorrhizal symbiosis. (A) Confocal images of R. irregularis colonization stained with wheat germ agglutinin (WGA)-conjugated Alexa Fluor 488 at 6 weeks post inoculation. Left, middle and right panels show normal arbuscule development in wild-type, no colonization in slr1 and occasional colonization events in slr1 mutant. Scale bars, 150 μm. (B) Internal root length colonization of slr1 mutants at 6 weeks post inoculation with R. irregularis. The experiment was repeated three times and representative data is shown. Error bars show standard errors. (C) Interactions between SLR1 and DIP1 in a Y2H assay. (D) Protein-protein interactions in N. benthamiana leaves revealed by BiFC. YFP fluorescence of leaves co-transformed with SLR1-YFPc (C-terminal half of YFP) or OsRAM1-YFPc and DIP1-YFPn (N-terminal half of YFP). No YFP fluorescence in OsRAM1-YFPn/YFPc, OsRAM1-YFPn/SLR1-YFPc, DIP-YFPn/YFPc and YFPn/SLR1-YFPc. (E) The MBP-DIP1 fusion protein interacts with His-SLR1 in pull-down assays using heterologously expressed proteins in E. coli. (F) A time course (10-40 DPI) of relative expression of DIP1 following R. irregularis inoculation. These experiments were replicated two times. Bars represent standard error. (G) Ink stained roots of rice reveal a greatly reduced level of infection in the DIP1 RNAi lines at 6 weeks post inoculation. Scale bars, 150 μm. (H) Quantification of R. irregularis colonization levels in 8 independent DIP1 rice RNAi lines at 6 weeks post inoculation (CK, empty vector control). (I) Interactions between DIP1, OsRAM1 and MtRAM1 are shown in a Y2H assay. (J) The MBP-DIP1 fusion protein can interact with His-OsRAM1 in pull-down assays with heterologously expressed proteins in E. coli. In (C, I): BD, binding domain; AD, activation domain. -LT, SD/-Leu-Trp; -LTHA, SD/-Leu-Trp-His-Ade. 30 mM 3-AT (3-aminotriazole) was used to suppress protein autoactivation.
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References

    1. Güimil S, Chang HS, Zhu T, et al. Proc Natl Acad Sci USA. 2005. pp. 8066–8070. - PMC - PubMed
    1. El Ghachtouli N, Martin-Tanguy J, Paynot M, et al. FEBS Lett. 1996. pp. 189–192. - PubMed
    1. Gutjahr C, Banba M, Croset V, et al. Plant Cell. 2008. pp. 2989–3005. - PMC - PubMed
    1. Sun TP. Curr Biol. 2011. pp. R338–R345. - PubMed
    1. Ueguchi-Tanaka M, Ashikari M, Nakajima M, et al. Nature. 2005. pp. 693–698. - PubMed

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