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. 2013 Nov 18:2013:615454.
doi: 10.1155/2013/615454. eCollection 2013.

Insights on the phytochemical profile (cyclopeptides) and biological activities of Calotropis procera latex organic fractions

Thiago Lustosa Jucá  1 , Márcio Viana Ramos  1 , Frederico Bruno Mendes Batista Moreno  1 , Mayara Patrícia Viana de Matos  1 , José Delano Barreto Marinho-Filho  2 , Renato Azevedo Moreira  3 , Ana Cristina de Oliveira Monteiro-Moreira  3
Affiliations

Insights on the phytochemical profile (cyclopeptides) and biological activities of Calotropis procera latex organic fractions

Thiago Lustosa Jucá et al. ScientificWorldJournal. .

Abstract

Calotropis procera is a medicinal plant whose pharmacological properties are associated with its latex. Here, the Calotropis procera latex fractions were investigated in an attempt to trace its phytochemical profile and measure its anti-inflammatory and toxicity activity. The crude latex was partitioned, yielding five fractions (49.4% hexane, 5.2% dichloromethane, 2.0% ethyl acetate, 2.1% n-butanol, and 41.1% aqueous). Phytochemical screening and spectroscopy analysis revealed that dichloromethane is the most chemically diverse fraction. Triterpenes were detected in both the hexane and dichloromethane fractions, while flavonoids were detected in the dichloromethane and ethyl acetate fractions. These fractions were cytotoxic to cancer cell lines (LD50 0.05 to 3.9 μ g/mL) and lethal to brine shrimp (LD50 10.9 to 65.7 μ g/mL). Reduced neutrophil migration in rats was observed in carrageenan-induced peritonitis for the dichloromethane (67%), ethyl acetate (56%), and aqueous (72%) fractions. A positive reaction with tolidine and ninhydrin suggested that cyclopeptides are in the ethyl acetate fraction. It is therefore concluded that Calotropis procera latex dichloromethane and ethyl acetate fractions exhibit both in vitro and in vivo activities as well as anti-inflammatory properties. Cyclopeptide detection is especially interesting because previous attempts to investigate these low-molecular cyclic amino acid sequences in C. procera have failed.

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Figures

Figure 1
Figure 1
Fingerprinting analysis for C. procera latex fractions through infrared spectroscopy (left panel) and 1H-RMN (right panel).
Figure 2
Figure 2
Cyclopeptides in ethyl acetate fraction from C. procera are confirmed by tolidine (a) and ninhydrin chemical detection (b).
See this image and copyright information in PMC

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