Desiccation tolerance in resurrection plants: new insights from transcriptome, proteome and metabolome analysis

Abstract

Most higher plants are unable to survive desiccation to an air-dried state. An exception is a small group of vascular angiosperm plants, termed resurrection plants. They have evolved unique mechanisms of desiccation tolerance and thus can tolerate severe water loss, and mostly adjust their water content with the relative humidity in the environment. Desiccation tolerance is a complex phenomenon and depends on the regulated expression of numerous genes during dehydration and subsequent rehydration. Most of the resurrection plants have a large genome and are difficult to transform which makes them unsuitable for genetic approaches. However, technical advances have made it possible to analyze changes in gene expression on a large-scale. These approaches together with comparative studies with non-desiccation tolerant plants provide novel insights into the molecular processes required for desiccation tolerance and will shed light on identification of orphan genes with unknown functions. Here, we review large-scale recent transcriptomic, proteomic, and metabolomic studies that have been performed in desiccation tolerant plants and discuss how these studies contribute to understanding the molecular basis of desiccation tolerance.

Keywords: desiccation tolerance; metabolomics; proteomics; resurrection plants; transcriptomics.

FIGURE 1

Comparative studies of desiccation tolerant and sensitive plants using omics approaches and integration of the data to identify target genes for generating drought tolerant plants.

FIGURE 2

Diagrammatic representation of the responses of resurrection plants to desiccation/rehydration as analyzed by omics approaches. Indicated are the main classes of transcripts/metabolites characteristic for the different stages of the dehydration/rehydration cycle.