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. 2014 Jan;7(1):223-227.
doi: 10.3892/etm.2013.1396. Epub 2013 Nov 8.

Effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats

Fei Ye  1 , Xu-Qin Chen  2 , Guan-Shui Bao  3 , Yin Hua  4 , Zhe-Dong Wang  2 , Yi-Chuan Bao  5
Affiliations

Effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats

Fei Ye et al. Exp Ther Med. 2014 Jan.

Abstract

The objective of this study was to analyze the changes in expression and the possible functions of interleukin-6 (IL-6) in electrical kindling of the basolateral amygdala (BLA) in epileptic rats. Bipolar electrodes were implanted into the BLA of Sprague-Dawley rats, and the rats were then subjected to chronic electrical stimulation through the electrodes to induce kindling. The seizure characteristics and behavioral changes of the rats were observed, and electroencephalograms were recorded during and following kindling. The IL-6 mRNA expression in the hippocampi of the rats was analyzed using semi-quantitative reverse transcription-polymerase chain reaction, and control and topiramate (TPM)-treated groups were compared. The mean time-period required for kindling was 13.50±3.99 days, and the afterdischarge duration (ADD) measured between 21,450 and 119,720 msec. The expression of IL-6 mRNA was significantly upregulated in the kindled rats. TPM was able to depress the seizures and decrease the IL-6 level in the kindled rats. In conclusion, IL-6 mRNA was upregulated in the hippocampi of epileptic rats, and IL-6 may have participated in the process of kindling.

Keywords: basolateral amygdale; epilepsy; interleukin-6; kindling; rat; topiramate.

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Figures

Figure 1
Figure 1
Discharge of epileptic seizures in the rat.
Figure 2
Figure 2
Electrophoresis of the reverse transcription polymerase chain reaction products of interleukin-6 (IL-6). Lane 1, blank control; lane 2, surgical control; lane 3, epilepsy; lane 4, topiramate (TPM); lane M: marker.
See this image and copyright information in PMC

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