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. 2013 Dec 12;8(12):e83253.
doi: 10.1371/journal.pone.0083253. eCollection 2013.

Genetic variability and population structure of the mushroom Pleurotus eryngii var. tuoliensis

Mengran Zhao  1 , Chenyang Huang  1 , Qiang Chen  1 , Xiangli Wu  1 , Jibin Qu  1 , Jinxia Zhang  1
Affiliations

Genetic variability and population structure of the mushroom Pleurotus eryngii var. tuoliensis

Mengran Zhao et al. PLoS One. .

Abstract

The genetic diversity of 123 wild strains of Pleurotus eryngii var. tuoliensis, which were collected from nine geographical locations in Yumin, Tuoli, and Qinghe counties in the Xinjiang Autonomous Region of China, was analysed using two molecular marker systems (inter-simple sequence repeat and start codon targeted). At the variety level, the percentage of polymorphic loci and Nei's gene diversity index for P. eryngii var. tuoliensis was 96.32% and 0.238, respectively. At the population level, Nei's gene diversity index ranged from 0.149 to 0.218 with an average of 0.186, and Shannon's information index ranged from 0.213 to 0.339 with an average of 0.284. These results revealed the abundant genetic variability in the wild resources of P. eryngii var. tuoliensis. Nei's gene diversity analysis indicated that the genetic variance was mainly found within individual geographical populations, and the analysis of molecular variance revealed low but significant genetic differentiation among local and regional populations. The limited gene flow (Nm = 1.794) was inferred as a major reason for the extent of genetic differentiation of P. eryngii var. tuoliensis. The results of Mantel tests showed that the genetic distance among geographical populations of P. eryngii var. tuoliensis was positively correlated with the geographical distance and the longitudinal distances (rGo = 0.789 and rLn = 0.873, respectively), which indicates that geographical isolation is an important factor for the observed genetic differentiation. Nine geographical populations of P. eryngii var. tuoliensis were divided into three groups according to their geographical origins, which revealed that the genetic diversity was closely related to the geographical distribution of this wild fungus.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Sampling sites of Pleurotus eryngii var. tuoliensis in China.
The Xinjiang Autonomous Region is highlighted in blue in the map of China shown in the upper left. WX, ZC, SB, SD, HS, and WT in the Yumin region are marked as red rotundities; KF and HJ in the Tuoli region are marked as blue rotundities; and AR in the Qinghe region is marked as a green rotundity.
Figure 2
Figure 2. Mantel tests between the genetic distance and the geographical parameters.
(a) Mantel test between Nei’s genetic distance and the geographical distance among populations. (b) Mantel test between Nei’s genetic distance and the latitudinal distance. (c) Mantel test between Nei’s genetic distance and the longitudinal distance. In 2a, 2b, and 2c, the X-axis represents the geographical parameter, and the Y-axis represents Nei’s genetic distances between the populations.
Figure 3
Figure 3. UPGMA dendrogram of the nine populations of P. eryngii var. tuoliensis based on Nei’s genetic distance.
The scale bar means the genetic distance.
See this image and copyright information in PMC

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