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. 2013;2(Spec Iss):S0022.
doi: 10.5702/massspectrometry.S0022. Epub 2013 Apr 15.

MALDI Imaging Mass Spectrometry-A Mini Review of Methods and Recent Developments

Cecilia Eriksson  1 , Noritaka Masaki  2 , Ikuko Yao  3 , Takahiro Hayasaka  2 , Mitsutoshi Setou  2
Affiliations

MALDI Imaging Mass Spectrometry-A Mini Review of Methods and Recent Developments

Cecilia Eriksson et al. Mass Spectrom (Tokyo). 2013.

Abstract

As the only imaging method available, Imaging Mass Spectrometry (IMS) can determine both the identity and the distribution of hundreds of molecules on tissue sections, all in one single run. IMS is becoming an established research technology, and due to recent technical and methodological improvements the interest in this technology is increasing steadily and within a wide range of scientific fields. Of the different IMS methods available, matrix-assisted laser desorption/ionization (MALDI) IMS is the most commonly employed. The course at IMSC 2012 in Kyoto covered the fundamental principles and techniques of MALDI-IMS, assuming no previous experience in IMS. This mini review summarizes the content of the one-day course and describes some of the most recent work performed within this research field.

Keywords: Imaging Mass Spectrometry; MALDI IMS; heat stabilization; sample preparation.

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Figures

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Fig. 1. Aqueous washing illustrated on a whole body mouse pup by MALDI TOF. (A) Comparison of peak number between unwashed and washed tissue. ∗=Student’s t test p-value <2.0×10−4. (B) Examples of images obtained across a whole body mouse pup, illustrating organ specific lipid expression in the negative ion mode. Figure reprint by permission, Angel et al. Anal. Chem. 7(84): 1557–1564, 2012.
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Fig. 2. Ion mobility separation of biomolecular ions detected from thin tumor tissue sections. A) Representative spectrum of peak-picked data acquired during an IMS imaging experiment. The lipid peaks are highlighted in red, while matrix-related ions are shown in blue. B) Drift plot of the separated ions. Ion image 1 shows the distribution of a background matrix ion. Ion images 2 (m/z 746.5) and 3 (m/z 746.6) show the distribution of different lipids in the tumor tissue. Lipid-related ions (highlighted in red) were separated from background ions (blue). Figure reprint by permission, Chughtai et al. J. Lip. Res. 54(2): 333–344, 2013.
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Fig. 3. Quantitation: structural overview of the quantitation software design. Figure reprint by permission, Källback et al. J. Proteomics 75(16): 4941–4951, 2012.
See this image and copyright information in PMC

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